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The MicroGrid at BIOGEM is designed for high throughput sample handling with the ability to work from 96 and 384 or 1536 well microplates. Up to 24 microplates at a time can be accommodated in the BioBank loading cartridge and a maximum of 10 BioBanks (240 microplates) may be programmed into a single run.
The MicroGrid is currently used to create both PCR based and oligonucleotide based custom microarrays on derivatized glass slides.
Dust can pose a challenge for the small diameter of the 10K pins: the spotter needs therefore to be located in a cleanroom environment. The 2500K pins are also utilized, and have less clogging problems due to their larger size. Respective spot images from both pins are shown.
Purpose: To determine the number of spots a 10K pin can print with one sample dip.
A pin refill test was carried out using cye 3 labeled genomic DNA in DMSO, and Corning Gap slides to determine how many spots a 10K pin can print with one sample dip. The subarray grid is 20 by 20. The Cy3 labelled DNA spots were still present after 400 contacts with the slide surface. Image generated by Colleen Eckhardt.
Last Updated March 2008
By Dr. Gary Hardiman
