biogem

Illumina

This can be used for genotyping services, which will be offered in the near future.

Illumina (San Diego, Ca) has developed a bead-based technology for SNP genotyping and gene expression profiling applications on two distinct substrates, the Sentrix LD BeadChip and the Sentrix Array Matrix (which facilitate up to 8 and 96 sample respectively). Both are fabricated to utilize an 'array of arrays' format, which enables the processing of multiple samples at the same time. Each array on each substrate contains thousands of tiny etched wells, into which thousands to hundreds of thousands of 3-micron beads self-assemble in a random fashion. 50-mer gene-specific probes concatenated with 'address or zip-code' sequences are immobilized on the bead surface. After bead assembly, each array is 'decoded' via a proprietary process, to determine which bead type containing which sequence, is present in each well of the substrate. The advantages of this platform are its sensitivity and reproducibility.

There are two genotyping assays that BIOGEM will support

GoldenGate® Genotyping - Linkage and fine mapping standard content panels and custom SNP panels from 96- to 1536-plex
Infinium™ Whole-Genome Genotyping - Integrated solutions for 100, 300 and 500K SNP loci.

Last Updated March 2009
By Dr. Gary Hardiman