biogem: Illumina Sentrix® Expression BeadChip Services

Sentrix® Expression BeadChips

Expression Services

BIOGEM provides Illumina expression analysis services to both UCSD and non-UCSD researchers. Investigators have the option of either having BIOGEM perform both the target labeling and chip processing, or having BIOGEM perform the chip processing with labeled target provided by the investigator. For optimal microarray data, researchers are encouraged to have BIOGEM perform the target labeling and hybridization.

The Biomedical Genomics Facility is fully equipped to process Illumina Sentrix® Expression BeadChips, once total RNA has been provided by the investigator.

The costs associated with an Illumina Sentrix Expression Beadchip experiment include

The cost of the Illumina Sentrix Expression BeadChips (at present two formats are available, permitting the analysis of six and eight samples respectively. The 6 Expression BeadChip can generate RNA profiles of six samples, analyzing over 46,000 transcripts per sample (i.e. full genome coverage). The 8 Expression BeadChip enables parallel analysis of more samples (eight in total) but less gene content (>23,000 transcripts).
The costs of the labeling reagents (this is currently $61 per sample for UCSD investigators, including biotin and all the ancillary reagents and materials). Depending on whether you are running an 8 or 6 Expression BeadChip, the cost will be $488 and $366 respectively.
Costs of the Illumina BeadChips. Illumina is currently providing UCSD with special pricing for Illumina BeadChips. Please contact Chad D. Geringer for details on pricing and any promotions.

Slides and Regents can be ordered directly from Illumina and shipped directly to BIOGEM.

Labor Costs

The labor cost is based on the number of biochips processed at the same time and whether BIOGEM carries out the labeling and hybridization or just the hybridization. For optimal data, researchers are strongly encouraged to have BIOGEM carry out the labeling and hybridization. The labor cost has a volume discount. For greater than 96 samples please contact Cyndy Illeman.

To generate an estimate of how much an Illumina BeadChip experiment costs, please use our Microarray Cost Calculator.

Note this is in addition to the cost of the Illumina biochips (which are ordered directly from Illumina). Please contact Chad D. Geringer for details.

Explanation of Rates

The UCSD rates are applicable to UCSD researchers. Service is however available to non-UCSD researchers.

For those researchers working at non-profit institutions (i.e. academic/research institutes) there is an outside non-profit rate. The rate is based on the UCSD rate plus 45%. CA Sales Tax (7.75%) will be collected from all Non-UC clients.

For those researchers working at for-profit institutions (i.e. biotech companies/pharma) there is an outside for-profit rate. The rate is based on the UCSD rate plus 100%. CA Sales Tax (7.75%) will be collected from all Non-UC clients.

Researchers that work at an outside institute but have a UCSD affiliation (e.g. Adjunct Appointment/collaboration) will only be eligible for the UCSD rate if payment is with a UCSD index number. If payment is via a PO from their respective organization then the outside non-profit or outside for-profit rate will apply. For more information, please contact Cyndy Illeman.

How to Order Biogem Illumina Expression Analysis Services

Read the instructions on how to proceed and fill out the online form.
Please order biochips from Illumina. Contact Chad D. Geringer for details. Biochips should be shipped directly to the attention of James Sprague at BIOGEM.
Prior to sending samples to BIOGEM, non-UC clients should provide Cyndy Illeman with a formal company Purchase Order including all terms with current billing address. This should be signed by official company purchasing agent. Payment should be made payable to UC REGENTS and ONLY after the UCSD Invoice is received by your accounting office. Payment should include remittance advice copy per invoice instructions. DO NOT SEND PAYMENT WITH PO. Final rate to be billed is at BIOGEM staff's determination. Billing questions should be directed to Cyndy Illeman.

Scientific questions, order production and/or performance comments should be directed to Dr. Gary Hardiman.

Last Updated March 2008
By Dr. Gary Hardiman