biogem: Illumina Sentrix® Expression BeadChip Services

LightCycler 480 Real-Time PCR System

LightCycler 480 Assay Costs

BIOGEM is currently supporting the Sybergreen and UPL formats for assays on the LightCycler 480 Real-Time PCR System. These services are offered to UCSD investigators and priority is given to investigators running microarray experiments at BIOGEM. Services are available to non-UCSD investigators working at both non profit institutions and biotech or pharmaceutical companies.

It is anticipated that other assays will be brought online during 2008.

For more details and volume discounts contact Colleen Eckhardt.

The costs associated with a LightCycler 480 experiment include:
The costs of the reagents. For RNA expression profiling using the Light Cycler 480, there are two components

RNA conversion into cDNA.
The reagent costs per RNA sample is 7$ (which includes the necessary reagents and ancillary reagents). This typically generates enough cDNA for 10 assays.
Real Time PCR on the cDNA using either the Sybergreen or universal probe library (UPL) approach. For a 96 well assay the cost is $80 per plate. For a 384 well assay the cost is $200.

This includes all the reagents and consumables, except the primers which must be supplied by the investigator.

If using the UPL approach the investigator has the choice of purchasing the Universal Probe from Roche or BIOGEM. Please contact Marcia Salvestrini for pricing if you plan on using large amounts of a particular probe (i.e. greater than 100 assays). If you plan on running a limited number of samples (i.e. less than 100) BIOGEM has the complete library (UPL) in its freezers and the cost per probe per 96 well experiment is $65.

Labor

For cDNA synthesis for up to 20 samples is $165. Please note this is a flat fee for cDNA synthesis that is the same whether 1 or 20 samples are submitted. Alternatively, the researcher may provide their own cDNA. To keep variability to a minimum, it is best if the cDNA is generated with the same protocol or kit.

For real-time PCR. The assays are offered in either 96 or 384 well plate formats. For a 96 well experiment the cost is $550. For a 384 well experiment the cost is $660. Please note that these rates are independent of the number of samples assayed. If just 10 samples are assayed in a 96 well assay, the cost remains $550. These labor rates include assay set up, the QPCR and preliminary data analysis.

Initial experimental consultation and data analyses services are available and this is billed at $100 per hour.

Rate Code	Rate	Explanation
cregLCsyn	$7	cDNA reagents per sample
cregLC96	$80	PCR costs per 96 well plate
cregLC384	$200	PCR costs per 384 well plate
UPLLC96	$60	upl reagent cost per 96 well exp
UPLLC384	$120	upl reagent cost per 384 well exp
clabLC96	$550	labor per 96 well experiment
clabLC384	$660	labor per 96 well experiment
clabLCsyn	$165	labor - cDNA synthesis per sample

Explanation of Rates

The UCSD rates are applicable to UCSD researchers.

For those researchers working at non-profit institutions (i.e. academic/research institutes) there is an outside non-profit rate. The rate is based on the UCSD rate plus 45%. CA Sales Tax (7.75%) will be collected from all Non-UC clients.

For those researchers working at for-profit institutions (i.e. biotech companies/pharma) there is an outside for-profit rate. The rate is based on the UCSD rate plus 100%. CA Sales Tax (7.75%) will be collected from all Non-UC clients. Researchers that work at an outside institute but have a UCSD affiliation (e.g. Adjunct Appointment/collaboration) will only be eligible for the UCSD rate if payment is with a UCSD index number. If payment is via a PO from their respective organization then the outside non-profit or outside for-profit rate will apply. For more information on payment, please contact Cyndy Illeman.

How to Order Biogem Roche Light Cycler 480 Services

Read the instructions on how to proceed and fill out the online form.
Prior to sending samples to BIOGEM, non-UC clients should provide Cyndy Illeman with a formal company Purchase Order including all terms with current billing address. This should be signed by official company purchasing agent. Payment should be made payable to UC REGENTS and ONLY after the UCSD Invoice is received by your accounting office. Payment should include remittance advice copy per invoice instructions. DO NOT SEND PAYMENT WITH PO. Final rate to be billed is at BIOGEM staff's determination. Billing questions should be directed to Cyndy Illeman.

Scientific questions, order production and/or performance comments should be directed to Dr. Gary Hardiman.

Last Updated March 2008
By Dr. Gary Hardiman