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The Universal Probe Library from Roche utilizes short hydrolysis probes of only 8 or 9 bases. The high probe-melting temperatures characteristic of longer probes are retained by using Locked Nucleic Acid (LNA) nucleotide chemistry in these shorter probes. As the probes are only 8 or 9 bases long, each probe can hybridize to over 7,000 transcripts; thus, a set of only 90 probes can enable the quantification of virtually any transcript in a transcriptome.
Assay specificity is attained through the combination of specific primers and the Universal Probe Library probe.
Specific, intron-spanning real-time qPCR assays for human, mouse, rat, Drosophila, Arabidopsis, C. elegans, and primates can be easily designed.
A highly specific, optimized assay overnight using pre-validated Universal ProbeLibrary probes. Choose individual probes or a complete set of 90 probes stored in your freezer.
